app-tg mice app23  (Novartis)

Journal: Alzheimer's Research & Therapy

Article Title: The presence of circulating human apolipoprotein J reduces the occurrence of cerebral microbleeds in a transgenic mouse model with cerebral amyloid angiopathy

doi: 10.1186/s13195-024-01541-5

Figure Lengend Snippet: ( A ) Representative T2*-weighted cerebral MRI sections of APP23 mice chronically treated with saline or rhApoJ and WT mice treated with saline. CMB are indicated with red arrows. Graphical representation of the total number of hemorrhagic lesions. ( B ) Graphical representation of the number of hemorrhagic lesions, CMB (50–300 μm diameter) and large hemorrhagic lesions (> 300 μm) in the cortex, and ( C ) in deep brain regions (thalamus and basal ganglia). ( D ) Comparison of cerebral hemorrhagic lesions in T2*-MRI and Prussian blue staining showing iron hemosiderin deposits. The scale bar represents 20 μm. Data are presented as the mean + SD. #: count; *: p < 0.05; **: p < 0.01; ***: p < 0.001

Article Snippet: APP23 C57BL/6 mice (B6.Cg-Tg (Thy1-APP) 3Somm/J) mice were obtained from The Jackson Laboratory (Bar Harbor, ME, USA) and C57BL/6 WT mice were obtained from Janvier Labs (Le Genest-Saint-Isle, France).

Techniques: Saline, Comparison, Staining

Journal: Alzheimer's Research & Therapy

Article Title: The presence of circulating human apolipoprotein J reduces the occurrence of cerebral microbleeds in a transgenic mouse model with cerebral amyloid angiopathy

doi: 10.1186/s13195-024-01541-5

Figure Lengend Snippet: ( A ) Representative images of immunofluorescence staining of fibrinogen in green from brain sections of APP23 mice chronically treated with saline or rhApoJ and WT mice. ( B ) Graphical quantification of fibrinogen-positive cerebral vessels. Correlation between the number of fibrinogen-positive vessels and the number of cerebral hemorrhagic lesions detected by T2*-MRI. ( C ) Representative images of immunohistochemical staining of sma in brown from brain sections of APP23 mice chronically treated with saline or rhApoJ and WT mice. ( D ) Graphical quantification of sma-positive cerebral vessels per mm 2 . Correlation between the number of sma-positive vessels and the number of hemorrhagic lesions. The scale bar represents 50 μm. Data are presented as boxplots. #: count; *: p < 0.05; **: p < 0.01; ***: p < 0.001

Article Snippet: APP23 C57BL/6 mice (B6.Cg-Tg (Thy1-APP) 3Somm/J) mice were obtained from The Jackson Laboratory (Bar Harbor, ME, USA) and C57BL/6 WT mice were obtained from Janvier Labs (Le Genest-Saint-Isle, France).

Techniques: Immunofluorescence, Staining, Saline, Immunohistochemical staining

Journal: Alzheimer's Research & Therapy

Article Title: The presence of circulating human apolipoprotein J reduces the occurrence of cerebral microbleeds in a transgenic mouse model with cerebral amyloid angiopathy

doi: 10.1186/s13195-024-01541-5

Figure Lengend Snippet: ( A ) Graphical quantification of plasma human ApoJ levels (hApoJ) (ng/mL) and representative image of human ApoJ immunodetection in a brain cortex section from a rhApoJ-treated APP23 mouse. A consecutive brain slice was stained with ThS to confirm the presence of CAA in the vessel. The scale bar represents 20 μm. ( B ) Graphical representation of plasma levels of Groα (pg/mL), MIP-1α (pg/mL), and MMP-12 (ng/mL) in mice from Group 2. Data are presented as boxplots. *: p < 0.05; ***: p < 0.001. ( C ) Correlation between plasma MMP-12 levels (ng/mL) and the number of large hemorrhagic lesions in the brain cortex (A) and the volume (mm 3 ) of cortical hemorrhagic lesions

Article Snippet: APP23 C57BL/6 mice (B6.Cg-Tg (Thy1-APP) 3Somm/J) mice were obtained from The Jackson Laboratory (Bar Harbor, ME, USA) and C57BL/6 WT mice were obtained from Janvier Labs (Le Genest-Saint-Isle, France).

Techniques: Clinical Proteomics, Immunodetection, Slice Preparation, Staining

Journal: International Journal of Molecular Sciences

Article Title: Statin Therapy and the Development of Cerebral Amyloid Angiopathy—A Rodent in Vivo Approach

doi: 10.3390/ijms17010126

Figure Lengend Snippet: Average numbers of cMBs in treated and untreated APP23-tg mice in total and categorized by size and analyzed via magnetic resonance imaging (MRI).

Article Snippet: Heterozygote B6,D2-TgN(Thy-APP SWE )-23-tg mice (APP23-tg) were provided by Matthias Staufenbiel (Novartis Institutes for BioMedical Research, Novartis Pharma AG, Basel, Switzerland) and backcrossed twice with C57BL/6 mice (Janvier, Saint Berthevin Cedex, France).

Techniques: Magnetic Resonance Imaging, Mouse Assay, Control